The spectroscopic content within optical coherence tomography (OCT) data can provide a wealth of information. Spectroscopic OCT has the ability to enhance the image contrast, which may be notably applicable to tissue pathology for differentiation of tissues having unique spectroscopic properties.  Spectroscopic OCT methods are frequently limited by time-frequency trade-offs that limit high spectral and spatial resolution simultaneously. We present spectroscopic spectral-domain optical coherence microscopy performed with a multimodality microscope. Restricting the spatial extent of the signal by using high-numerical-aperture optics makes high-resolution spectroscopic information accessible, facilitated with spectral-domain detection. Simultaneous acquisition of multiphoton microscopy images is used to validate tissue structure and localization of nuclei within individual cells.

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