The differential-interference-contrast (DIC) microscope is of widespread use in life sciences as it enables noninvasive visualization of transparent objects. The goal of this work is to model the image formation process of thick three-dimensional …
We reply to a comment by Wang et al. [J. Opt. Soc. Am. A28, 662 (2011)10.1364/JA.28.000662] on the paper of Trattner et al.[J. Opt. Soc. Am. A26, 1147 (2009)10.1364/JOSAA.26.001147]. Although we agree with some of the points raised, we show that …
The need for the reconstruction and quantification of visualized objects from light microscopy images requires an image formation model that adequately describes the interaction of light waves with biological matter. Differential interference …