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Dr. Lisa E. Freed,
M.D., Ph.D.
SELECTED PUBLICATIONS (in reverse chronological order, representative
of 80 papers, 11 book chapters, 124 abstracts, 6 patents/patent applications)
Ye, X., Lu, L., Kolewe, M.E., Hearon,
K., Fisher, K.M., Coppeta, J., Freed, L.E. (2014). Scalable Unit for Building Cardiac Tissue.
Advanced Materials, Sep 19, 2014 E-Pub ahead of print, DOI:
10.1002/adma.201403074.
Park, H., Larson, B.L., Kolewe, M.E.,
Vunjak-Novakovic, G., Freed, L.E.
(2014). Biomimetic Scaffold Combined with Electrical Stimulation and Growth
Factor Promotes Tissue Engineered Cardiac Development. Experimental Cell
Research, 321(2) 297-306. PMC3946629.
Ye, X., Lu, L., Kolewe, ME, Park, H, Larson, BL,
Kim, E, Freed, LE (2013). A
Biodegradable Microvessel Scaffold as a Framework
to Enable Vascular Support of Engineered Tissues Biomaterials, 34(38): 10007-10015,
PMC3899884.
Kolewe, ME, Park, H, Gray, C, Ye, X, Langer, R, Freed, L.E. (2013) 3D Structural
Patterns in Scalable Elastomeric Polymer Scaffolds Guide Engineered Tissue
Architecture. Advanced Materials, 25(32): 4459-4465, PMC3954574, (inside cover
of issue).
Neal, R.A., Jean, A., Park, H., Wu, P.B., Hsiao, J., Engelmayr,
G.C., Jr., Langer, R., and Freed, L.E. (2013):
Three-dimensional elastomeric scaffolds designed with cardiac-mimetic
structural and mechanical features. Tissue Eng
Part A. 19(5-6):793-807. PMC3566675.
Park, H., Larson, B.L., Guillemette, M.D., Jain,
S.R., Hua, C., Engelmayr, G.C., Jr., and
Freed, L.E. 2011. The significance of pore microarchitecture in a
multi-layered elastomeric scaffold for contractile cardiac muscle constructs.
Biomaterials. 32:1856-64. 21144580. PMC3030129.
Freed, L.E., Engelmayr, G.C., Borenstein, J.T., Moutos, F.T.,
Guilak, F. (2009): Advanced material strategies for
tissue engineering scaffolds. Advanced Materials 21(32-33):
3410-3418 (inside cover of issue). PMC3003664.
Cheng, M.Y., Moretti, M., Engelmayr,
G.C., Freed, L.E. (2009): Insulin-like growth factor-I and
slow, bi-directional perfusion enhance the formation of tissue-engineered
cardiac grafts. Tissue Engineering, 15(3): 645-653. PMC2810276
Engelmayr, G.C., Cheng, M., Bettinger,
C.J., Borenstein, J.T., Langer, R., Freed,
L.E. (2008): Accordion-like honeycombs for tissue engineering of
cardiac anisotropy. Nature Materials, 7: 1003-1010 (cover of
issue, with commentary on pp. 932-933). PMC2613200.
Radisic M., Park, H., Martens, T.P.,
Salazar-Lazaro, J.E., Geng, W., Wang, Y., Langer,
R., Freed, L.E., Vunjak-Novakovic, G. (2008): Pre-treatment
of synthetic elastomeric scaffolds by cardiac fibroblasts improves engineered
heart tissue. J Biomedical Materials Research-A
86: 713-724.PMC2775086.
Nichol, J. W., Engelmayr, G. C., Cheng, M., and Freed,
L. E. (2008): Co-culture induces alignment in engineered cardiac
constructs via MMP-2 expression. Biochemical Biophysical Research Comm 373: 360-365. PMC2577781.Â
Augst, A., Marolt, D.,
Freed, L.E. , Vepari, C., Meinel, L., Farley, M., Fajardo, R., Patel, N., Gray,
M.L., Kaplan, D.L., Gordana Vunjak-Novakovic, G. (2008): Effects of chondrogenic and osteogenic
regulatory factors on composite constructs grown using human bone marrow stem
cells, silk scaffolds and bioreactors. J of the Royal Society Interface
5: 929-939. PMC2607468.
Freed, L.E., Guilak, F. (2007):
“Engineering functional tissues.” In: Principles of Tissue Engineering 3rd
edition, Ch.11 (R.P. Lanza, R. Langer and J. Vacanti,
eds., Academic Press). Ch. 11, pp. 137-153.
Moutos, F.T., Freed, L.E., Guilak, F. (2007): A biomimetic
three-dimensional woven composite scaffold for functional tissue engineering
of cartilage. Nature Materials 6:162-167 (cover of issue, with
commentary pp. 89-90).
Cheng, M. Y., Park, H., Engelmayr, G. C., Moretti, M., and Freed, L. E. (2007):
Effects of regulatory factors on engineered cardiac tissue in vitro.
Tissue Engineering 13: 2709-2719
Freed, L.E., Guilak, F., Guo, X.E., Gray, M.L., Tranquillo, R., Holmes, J., Radisic, M., Sefton, M.,
Kaplan, D., Vunjak-Novakovic, G. (2006): Advanced tools: scaffolds,
bioreactors, and signaling, Tissue Engineering 12: 3285- 3305 (cover
of issue)
Marolt, D., Augst, A., Freed,
L.E., Vepari, C., Fajardo, R., Patel, N.,
Gray, M., Farley, M., Kaplan, D., Vunjak-Novakovic, G. (2006): Bone and
cartilage tissue constructs grown using human bone marrow stromal cells, silk
scaffolds, and rotating bioreactors. Biomaterials 27(36):6138-6149
Radisic, M, Park, H, Chen, F, Wang, Y, Dennis,
R, Langer, R, Freed, LE , Vunjak-Novakovic, G. (2006): Biomimetic
approach to cardiac tissue engineering: oxygen carriers and channeled
scaffolds. Tissue Engineering, 12(8): 1-15 (cover of issue)
Boublik J., Park, H., Radisic,
M., Tognana, E., Chen, F., Pei ,
M., Vunjak-Novakovic, G., Freed, L.E. (2005): Mechanical
properties and remodeling of hybrid cardiac constructs made from heart cells,
fibrin, and a biodegradable elastomeric knitted fabric. Tissue
Engineering 11 (7-8): 1122-1132
Tognana, E., Padera,
R.F., Chen, F., Vunjak-Novakovic, G., Freed, L.E. (2005):
Development and remodeling of engineered cartilage-explant composites in
vitro and in vivo. Osteoarthritis and Cartilage 13(10): 896-905
Tognana, E., Chen, F., Padera,
R.F., Leddy, H.A., Christensen, S.E., Guilak, F., Vunjak-Novakovic, G., Freed, L.E.
(2005): Adjacent tissue (cartilage, bone) affect the functional integration
of engineered calf cartilage in vitro. Osteoarthritis and
Cartilage 13(2): 129-138
Schäfer, D., Seidel, J.O, Martin, I., Jundt, G., Heberer, M., Grodzinsky, A.L., Vunjak-Novakovic, G., Freed,
L.E. (2004): Engineering and characterization of functional osteochondral repair tissue. Orthopäde
33: 721–726
Radisic, M., Park, H. Shin, H., Consi,
T., Schoen, F.J., Freed, L.E., Vunjak-Novakovic, G. Novakovic G. (2004): Functional assembly of engineered
myocardium by electrical stimulation of cardiac myocytes
cultured on scaffolds. Proc. National Acad of
Sciences USA 101: 18129-18134.
Radisic, M., Yang, L, Boublik,
J, Cohen, R.J., Langer, R., Freed, L.E., Vunjak-Novakovic,
G. (2004): Medium perfusion enables engineering of compact and contractile
cardiac tissue. Am J Physiology (Heart & Circulatory Physiol.)
286: H507-516.
Bursac, N., Papadaki,
M., White, J.A., Eisenberg S.R., Vunjak-Novakovic, G., Freed, L.E. (2003):
Cultivation in rotating bioreactors promotes maintenance of cardiac myocyte electrophysiology and molecular properties, Tissue
Engineering, 9(6): 1243-1253
Freed, L.E., Rupnick, M.A., Schaefer, D.,
Vunjak-Novakovic, G. (2003): “Engineering functional cartilage and cardiac
tissue: in vitro culture parameters”. In: Functional Tissue
Engineering: the Role of Biomechanics (F. Guilak,
D. Butler, D. Mooney, S. Goldstein, eds, Springer Verlag) Ch. 27, pp. 360-376.
Seidel, J.O., Pei, M., Gray, M.L., Langer, R, Freed, L.E.,
Vunjak-Novakovic, G. (2004): Long term culture of tissue engineered cartilage
in a perfused chamber with mechanical stimulation. Biorheology
41: 445-458
Freed, L.E .
and G. Vunjak-Novakovic, (2002)
“Cell-Polymer-Bioreactor Systems”. In: Methods of Tissue Engineering (A.
Atala and R.P. Lanza, eds. Academic Press) Ch. 6,
pp. 99-114.
Freed, L.E. and G. Vunjak-Novakovic (2002) “Spaceflight bioreactor studies
of cells and tissues”. In: Cell Biology and Biotechnology in Space ( A.
Cogoli, ed., Elsevier Science) Advances in
Space Biology and Medicine Ch. 8, pp. 177-195.
Vunjak-Novakovic, G., Searby, N.D. de Luis, J., Freed, L.E. (2002):
Microgravity studies of cells and tissues. Ann. N.Y. Acad. Sci.
974: 504-517
Schaefer, D., Martin, I., Jundt, G., Seidel, J., Heberer, M., Grodzinsky, A.J,
Bergin, I., Vunjak-Novakovic, G., Freed,
L.E. (2002): Tissue engineered composites for the repair of large osteochondral defects. Arthritis and Rheumatism,
46: 2524-2534
Pei, M., Solchaga, L.A., Seidel, J., Zeng, L,
Vunjak-Novakovic, G., Caplan, A.I., Freed,
L.E. (2002): Bioreactors mediate the effectiveness of tissue
engineering scaffolds, FASEB J. 16: 1691-1694
Papadaki, M., Bursac, N.,
Langer, R., Merok, J., Vunjak-Novakovic, G., Freed,
L.E. (2001): Tissue engineering of functional cardiac muscle:
molecular, structural and electrophysiological studies, Am J Physiology
(Heart & Circulatory Physiol.) 280: H168–H178, 2001 (cover
of issue)
Martin, I., Shastri, V.P., Padera,
R.F., Yang, J., McKay, A.J., Langer, R., Vunjak Novakovic, G., Freed, L.E. (2001):
Selective differentiation of bone marrow stromal cells cultured on
three dimensional polymer foams J. Biomedical Materials Research
55: 229-235
Gooch, K.J., Blunk, T., Courter, D.L., Sieminski, A.L., Bursac, P.M.,
Vunjak-Novakovic, G, Freed, L.E. (2001): IGF-I and
mechanical environment interact to modulate engineered cartilage development,
Biochem. Biophys.
Res. Comm., 286: 909-915
Schaefer, D., Martin, I., Shastri, P., Padera, R.F., Langer, R., Freed, L.E.,
Vunjak-Novakovic, G. (2000): In vitro generation of osteochondral
composites. Biomaterials 21: 2599-2606
Freed, L.E .
and Vunjak-Novakovic, G. (2000). “Tissue Engineering
of Cartilage”. In: The Biomedical Engineering Handbook, 2nd edition,
(J.D. Bronzino, ed., CRC Press) Vol. II, pp. 124-1
– 124-26.
Freed, L.E .
and Vunjak-Novakovic, G. (2000). “Tissue Engineering
Bioreactors”. In: Principles of Tissue Engineering, 2 nd edition (R.P. Lanza, R. Langer and J. Vacanti, eds., Academic Press) Ch. 13, pp. 143-156.
Bursac N., Papadaki
M., Cohen R.J., Schoen F.J., Eisenberg S., Carrier R., Vunjak-Novakovic G., Freed,
L.E. (1999): Cardiac muscle tissue engineering: toward an in vitro
model for electrophysiological studies, Am. J Physiol
(Heart & Circulatory Physiology 46) 277: H433-H444
Freed L.E., Pellis N., Searby N., de Luis J., Preda C., Bordonaro J.,
Vunjak-Novakovic G. (1999) Microgravity Cultivation of Cells and Tissues. Gravitational
and Space Biology Bulletin 12: 57-66.
Martin, I., Vunjak-Novakovic, G., Yang, J., Langer, R., Freed,
L.E. (1999): Mammalian chondrocytes expanded in the
presence of fibroblast growth factor-2 maintain the ability to differentiate
and regenerate three-dimensional cartilaginous tissue. Experimental Cell
Research 253: 681 - 688
Obradovic B, Carrier R.L., Vunjak-Novakovic G.V., Freed,
L.E. (1999): Gas exchange is essential for bioreactor cultivation of
tissue engineered cartilage, Biotechnology& Bioengineering,
63:197-205
Vunjak-Novakovic, G., Martin, I., Obradovic, B., Treppo S., Grodzinsky, A.J.,
Langer R., Freed, L.E. (1999): Bioreactor
cultivation conditions modulate the composition and mechanical properties of
tissue engineered cartilage. Journal of Orthopedic Research 17,
130-138
Martin, I, Padera, R.F., Vunjak-Novakovic, G., Freed,
L.E. (1998): In vitro differentiation of chick embryo bone marrow
stromal cells into cartilaginous and bone-like tissues, J. Orthopaedic Research 16: 181-189
Freed, L.E., Hollander, A.P., Martin, I., Barry, J.R.,
Martin, I., Vunjak-Novakovic, G. (1998): Chondrogenesis
in a cell-polymer-bioreactor system, Experimental Cell Research 240:
58-65
Vunjak-Novakovic G., Obradovic B., Bursac P., Martin, I., Langer R., Freed L.E.
(1998): Dynamic seeding of polymer scaffolds for cartilage tissue
engineering, Biotechnology Progress 14: 193-202.
Freed, L.E., Langer, R., Martin, I., Pellis,
N., Vunjak-Novakovic, G. (1997): Tissue engineering of cartilage in space, Proceedings
of the National Academy of Sciences USA, 94: 13885-13890 (with
commentary, pp. 13380-13382)
Freed, L.E., Vunjak-Novakovic, G (1997). Microgravity
Tissue Engineering, In Vitro Cellular and Developmental Biology 33:
381-385.
Vunjak-Novakovic, G., Freed, L.E., Biron
R., Langer, R. (1996). Effects of mixing on tissue engineered cartilage,
Journal of the American Institute of Chemical Engineers 42 (3) 850-860.
Langer, R., Vacanti, J.P., Vacanti,
C., Atala, A., Freed, L.E., Vunjak-Novakovic, G. (1995): Tissue engineering:
biomedical applications, Tissue Engineering 1: 151-161.
Freed L.E., Vunjak-Novakovic, G (1995). Cultivation of
Cell-Polymer Constructs in Simulated Microgravity, Biotechnology and
Bioengineering 46: 306-313.
Freed, L.E., Grande, D.A., Emmanual,
J., Marquis, J.C., Lingbin, Z., Langer, R. (1994):
Joint resurfacing using allograft chondrocytes and synthetic biodegradable
polymer scaffolds, J. Biomedical Materials Research, 28: 891-899.
Freed, L.E., Vunjak-Novakovic, G., Biron,
R.J., Eagles, D., Lesnoy, D., Barlow, S.K., Langer,
R. (1994): Biodegradable polymer scaffolds for tissue engineering, Nature
Biotechnology 12: 689-693.
Freed, L.E., Vunjak-Novakovic G., Langer, R. (1993)
Cultivation of cell-polymer cartilage implants in bioreactors, J. Cell
Biochemistry 51, 257-264
Freed, L.E., G. Vunjak-Novakovic, P.A. Drinker and R.
Langer. (1993): A bioreactor based on suspended particles of immobilized
enzyme, Ann. Biomedical Engineering 21, 57-65
Freed, L.E., Marquis, J., Nohria,
A., Mikos, A.G., Emmanual,
J., Langer, R. (1993): Neocartilage formation in
vitro and in vivo using cells cultured on synthetic biodegradable polymers, J
Biomedical Materials Research 27:11-23 (cover of issue)
Freed, L.E., Vunjak-Novakovic, G., Bernstein, H., Cooney
C.L., Langer, R. The Kinetics of Immobilized Heparinase
in Human Blood, Annals of Biomedical Engineering 21, 67-76, 1993
Freed, L.E. ,
G. Vunjak-Novakovic, P.A. Drinker and R. Langer. (1993): A Bioreactor Based on
Suspended Particles of Immobilized Enzyme, Annals of Biomedical
Engineering 21, 57-65
Freed, L.E., Endemann, G., Tomera, J.F., Gavino, V.C., Brunengraber, H. (1988): Lipogenesis
from Ketone Bodies in Perfused Livers of Streptozotocin
Diabetic Rats, Diabetes 37: 50-55
Kopito, R., Weinstock, S., Freed, L.E., Murray,
D., Brunengraber, H. (1982): Metabolism of Plasma Mevalonate in Rats and Humans, Journal of Lipid
Research 23: 577-583
Freed, L.E., Freed, C., O'Donnell, R. (1982): Small
Signal Gain Saturation Intensity of I & II Vibrational Band Transitions
in Sealed-off CO2 Lasers, Journal of Quantum Electronics, 18:
1229-1236
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