David T. Wang
GRADUATE STUDENT · MIT/WHOI · GEOCHEMISTRY

Hi! Thanks for stopping by.

I am a Ph.D. candidate in the MIT/WHOI Joint Program. I work in the Hardcore Stable Isotope Laboratory at MIT. My Ph.D. advisor is Shuhei Ono.

research

Short descriptions of my current and past projects are below.

THE GEOCHEMISTRY OF 13CH3D

Development and refinement of methods to measure 13CH3D, a clumped isotopologue of methane, in gases sourced from near-surface sediments, sedimentary basins, and crystalline rocks. Analytical work and project development is at MIT with Shuhei Ono. Field sampling and experimental work is done in collaboration with research groups at various institutions in multiple countries.

SULFUR ISOTOPE FRACTIONATION DURING MICROBIAL SULFATE REDUCTION

This project aims to test the controls exerted by organic matter quality and metabolic rates on sulfur isotope signatures in the rock record. This is work done at MIT with Shuhei Ono and Tanja Bosak.

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TRACE METALS, PROTEOMICS, AND MARINE HETEROTROPHY

Experiments on marine heterotrophic bacteria to test the influence of trace metals on rates of organic matter degradation in ocean. Shipboard experiments/sampling on "Metzyme", a month-long research expedition aboard the R/V Kilo Moana, which transected the equatorial Pacific from Hawaii to Samoa to collect a full-depth trace metal and metaproteome profile. This was work supervised by Mak Saito at WHOI.

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ISOTOPES AS TRACERS OF HEXAVALENT CHROMIUM CONTAMINATION

As an undergraduate at Cal State L.A., I worked with Andre Ellis to develop chromium stable isotopes as tracers of chromium contamination in groundwater. We measured isotopic fractionation during the oxidation of chromium(III) to (VI) by environmentally-prevalent manganese oxides. The results may be of use in the application of chromium isotopes for monitoring remediation of contaminated sites.

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MOLECULAR BIOLOGY OF PLATINUM RESISTANCE IN OVARIAN CANCER

I studied ovarian cancer cells with Oliver Dorigo and Chintda Santiskulvong at UCLA as a summer intern through the Amgen Scholars Program. Experimental work in support of a project to characterize the regulation of cellular resistance to cisplatin, a platinum-based chemotherapy agent. Approach involved gene expression profiling to first identify candidate pathways involved in cisplatin-resistance, and then cell-culture & protein analysis to characterize the possible role of one of the molecules identified, the insulin-like growth factor 1 receptor.

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publications + presentations

PUBLICATIONS

  1. Ono S, Wang DT, Gruen DS, Sherwood Lollar B, Zahniser M, McManus BJ, Nelson DD (2014) Measurement of a Doubly-Substituted Methane Isotopologue, 13CH3D, by Tunable Infrared Laser Direct Absorption Spectroscopy. Analytical Chemistry, in press. // doi:10.1021/ac5010579
  2. Sim MS, Wang DT, Zane GM, Wall JD, Bosak T, and Ono S (2013) Fractionation of sulfur isotopes by Desulfovibrio vulgaris mutants lacking hydrogenases or type I tetraheme cytochrome c3. Frontiers in Microbiology 4:171. // PDF // doi:10.3389/fmicb.2013.00171

PRESENTATIONS

Some talks & posters given at national or regional conferences.

  1. Wang DT, Gruen DS, Seewald JS, Whelan JK, Sherwood Lollar B, and Ono S (2014) Clumped Isotopic (13CH3D) Composition of Methane from the Potato Hills Gas Field, Oklahoma. Goldschmidt, Sacramento, CA. (talk)
  2. Wang DT, Ono S, and Bosak T (2014) Sulfur isotope fractionation during reduction of sulfate by Desulfovibrio fructosovorans. Northeastern Geobiology Symposium, Yale University. (talk)
  3. Wang DT, Fregoso DC, Ellis AS, Johnson TM, and Bullen TD (2010) Stable isotope fractionation during chromium(III) oxidation by δ-MnO2. AGU Fall Meeting, 2010. (poster)